microarray scanner tecan ls reloaded tm  (Tecan Systems)

Journal: International Journal of Environmental Research and Public Health

Article Title: Molecular Detection of a Potentially Toxic Diatom Species

doi: 10.3390/ijerph120504921

Figure Lengend Snippet: ( A ) Schematic overview of a microarray slide consisting of two blocks. Each block has four identical grids of probes; ( B ) Glass slide during loading procedure of a hybridization mixture; ( C ) Visualization of an hybridized slide after scanning: the green spots indicate the Cy3-Marker spotted at the four corners of the slide; the red spots indicate the positions where a fluorescent fragment of Amphora coffeaeformis SIT gene recognized the complementary oligonucleotide probes; the green square indicates one of the four sectors of one block.

Article Snippet: Fluorescence scanning of each slide was performed with a microarray scanner (TECAN LS Reloaded TM Microarray Scanner) for the spots corresponding to samples and markers, at 635 nm and at 532 nm, respectively.

Techniques: Microarray, Blocking Assay, Hybridization, Marker

Journal: International Journal of Environmental Research and Public Health

Article Title: Molecular Detection of a Potentially Toxic Diatom Species

doi: 10.3390/ijerph120504921

Figure Lengend Snippet: DNA sequence of the fragments of elongation factor eEF1-a ( A ) and SIT ( B ) obtained by PCR amplification from Amphora coffeaeformis chromosomal DNA. The sense and anti-sense probes specifically designed on this sequence and used for microarray analysis are indicated with black arrows and grey arrows, respectively.

Article Snippet: Fluorescence scanning of each slide was performed with a microarray scanner (TECAN LS Reloaded TM Microarray Scanner) for the spots corresponding to samples and markers, at 635 nm and at 532 nm, respectively.

Techniques: Sequencing, Amplification, Microarray

Journal: International Journal of Environmental Research and Public Health

Article Title: Molecular Detection of a Potentially Toxic Diatom Species

doi: 10.3390/ijerph120504921

Figure Lengend Snippet: Quantification results of microarray hybridization experiments targeting SIT gene of Amphora coffeaeformis . On the Y-axis are reported the probes producing a fluorescent signal after hybridization: yellow bars correspond to probes displaying high specificity, green bars indicate probes with lower specificity, while gray bars correspond to probes producing false-positive signals. The fluorescence intensity signals are reported on the histogram bars.

Article Snippet: Fluorescence scanning of each slide was performed with a microarray scanner (TECAN LS Reloaded TM Microarray Scanner) for the spots corresponding to samples and markers, at 635 nm and at 532 nm, respectively.

Techniques: Microarray, Hybridization, Fluorescence